free ca 2 Search Results


rabbit polyclonal anti xbp1  (Novus Biologicals)
92
Novus Biologicals rabbit polyclonal anti xbp1
Rabbit Polyclonal Anti Xbp1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/free+ca+2/Carbonic+Anhydrase+II%2FCA2+Antibody+-+BSA+Free/pmc09033049-35-38-42
Average 92 stars, based on 1 article reviews
rabbit polyclonal anti xbp1 - by Bioz Stars, 2026-11
92/100 stars
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tnfα neutralizing antibody infliximab  (Novus Biologicals)
92
Novus Biologicals tnfα neutralizing antibody infliximab
Tnfα Neutralizing Antibody Infliximab, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/free+ca+2/TNF-alpha+Antibody+(cA2+(Infliximab))+-+Chimeric+-+Azide+and+BSA+Free/pmc11361748-289-7-11
Average 92 stars, based on 1 article reviews
tnfα neutralizing antibody infliximab - by Bioz Stars, 2026-11
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infliximab  (Novus Biologicals)
92
Novus Biologicals infliximab
WRS increases MMP activity in macrophage-conditioned media by a TNFα-dependent pathway. PMA-differentiated THP1-derived macrophages were treated for 24 h with recombinant human WRS (100 nm), Δ1–47 WRS (100 nm) as an analog of the MMP7 and MMP8 cleavage product, TNFα (40 ng/ml), or buffer with or without a mAb inhibitor of TNFα <t>(infliximab</t> (IN)) (100 ng/ml). MMPs in 20-fold–concentrated conditioned media were activated with 1 mm APMA, and cleavage of the quenched fluorescence peptide substrate Mca-Pro-Leu-Gly↓Leu-Dpa-Ala-Arg-NH2 was measured (scatter plots show means ± S.D., n = 3) in N = 3 independent experiments. Statistical significance was determined against buffer + infliximab using a one-way ANOVA with Dunnett's multiple comparison posttests. ***, p < 0.001; ns, not significant. Error bars represent S.D.
Infliximab, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/free+ca+2/TNF-alpha+Antibody+(cA2+(Infliximab))+-+Chimeric+-+Azide+and+BSA+Free/pmc06721938-413-39-40
Average 92 stars, based on 1 article reviews
infliximab - by Bioz Stars, 2026-11
92/100 stars
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primary antibodies against ncx1  (Alomone Labs)
94
Alomone Labs primary antibodies against ncx1
WRS increases MMP activity in macrophage-conditioned media by a TNFα-dependent pathway. PMA-differentiated THP1-derived macrophages were treated for 24 h with recombinant human WRS (100 nm), Δ1–47 WRS (100 nm) as an analog of the MMP7 and MMP8 cleavage product, TNFα (40 ng/ml), or buffer with or without a mAb inhibitor of TNFα <t>(infliximab</t> (IN)) (100 ng/ml). MMPs in 20-fold–concentrated conditioned media were activated with 1 mm APMA, and cleavage of the quenched fluorescence peptide substrate Mca-Pro-Leu-Gly↓Leu-Dpa-Ala-Arg-NH2 was measured (scatter plots show means ± S.D., n = 3) in N = 3 independent experiments. Statistical significance was determined against buffer + infliximab using a one-way ANOVA with Dunnett's multiple comparison posttests. ***, p < 0.001; ns, not significant. Error bars represent S.D.
Primary Antibodies Against Ncx1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/free+ca+2/Anti-NCX1+(SLC8A1)+Antibody+-+Carrier+Free/pmc11342557-181-17-27
Average 94 stars, based on 1 article reviews
primary antibodies against ncx1 - by Bioz Stars, 2026-11
94/100 stars
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anti carbonic anhydrase ii  (Novus Biologicals)
90
Novus Biologicals anti carbonic anhydrase ii
WRS increases MMP activity in macrophage-conditioned media by a TNFα-dependent pathway. PMA-differentiated THP1-derived macrophages were treated for 24 h with recombinant human WRS (100 nm), Δ1–47 WRS (100 nm) as an analog of the MMP7 and MMP8 cleavage product, TNFα (40 ng/ml), or buffer with or without a mAb inhibitor of TNFα <t>(infliximab</t> (IN)) (100 ng/ml). MMPs in 20-fold–concentrated conditioned media were activated with 1 mm APMA, and cleavage of the quenched fluorescence peptide substrate Mca-Pro-Leu-Gly↓Leu-Dpa-Ala-Arg-NH2 was measured (scatter plots show means ± S.D., n = 3) in N = 3 independent experiments. Statistical significance was determined against buffer + infliximab using a one-way ANOVA with Dunnett's multiple comparison posttests. ***, p < 0.001; ns, not significant. Error bars represent S.D.
Anti Carbonic Anhydrase Ii, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/free+ca+2/Carbonic+Anhydrase+II%2FCA2+Antibody+-+BSA+Free/pm25487411-74-42-46
Average 90 stars, based on 1 article reviews
anti carbonic anhydrase ii - by Bioz Stars, 2026-11
90/100 stars
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hanks’ balanced salt solution with ca 2+ and 2  (Corning Life Sciences)
90
Corning Life Sciences hanks’ balanced salt solution with ca 2+ and 2
WRS increases MMP activity in macrophage-conditioned media by a TNFα-dependent pathway. PMA-differentiated THP1-derived macrophages were treated for 24 h with recombinant human WRS (100 nm), Δ1–47 WRS (100 nm) as an analog of the MMP7 and MMP8 cleavage product, TNFα (40 ng/ml), or buffer with or without a mAb inhibitor of TNFα <t>(infliximab</t> (IN)) (100 ng/ml). MMPs in 20-fold–concentrated conditioned media were activated with 1 mm APMA, and cleavage of the quenched fluorescence peptide substrate Mca-Pro-Leu-Gly↓Leu-Dpa-Ala-Arg-NH2 was measured (scatter plots show means ± S.D., n = 3) in N = 3 independent experiments. Statistical significance was determined against buffer + infliximab using a one-way ANOVA with Dunnett's multiple comparison posttests. ***, p < 0.001; ns, not significant. Error bars represent S.D.
Hanks’ Balanced Salt Solution With Ca 2+ And 2, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/free+ca+2/ca2++mg2++free+pbs/pmc09802210-146-0-29
Average 90 stars, based on 1 article reviews
hanks’ balanced salt solution with ca 2+ and 2 - by Bioz Stars, 2026-11
90/100 stars
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hbss without ca2 and mg2  (Biochrom)
90
Biochrom hbss without ca2 and mg2
WRS increases MMP activity in macrophage-conditioned media by a TNFα-dependent pathway. PMA-differentiated THP1-derived macrophages were treated for 24 h with recombinant human WRS (100 nm), Δ1–47 WRS (100 nm) as an analog of the MMP7 and MMP8 cleavage product, TNFα (40 ng/ml), or buffer with or without a mAb inhibitor of TNFα <t>(infliximab</t> (IN)) (100 ng/ml). MMPs in 20-fold–concentrated conditioned media were activated with 1 mm APMA, and cleavage of the quenched fluorescence peptide substrate Mca-Pro-Leu-Gly↓Leu-Dpa-Ala-Arg-NH2 was measured (scatter plots show means ± S.D., n = 3) in N = 3 independent experiments. Statistical significance was determined against buffer + infliximab using a one-way ANOVA with Dunnett's multiple comparison posttests. ***, p < 0.001; ns, not significant. Error bars represent S.D.
Hbss Without Ca2 And Mg2, supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/free+ca+2/ca2+++and+mg2++free+phosphate+buffered+saline++pbs+/10__1128_slash_aac__01018___07-57-21-22
Average 90 stars, based on 1 article reviews
hbss without ca2 and mg2 - by Bioz Stars, 2026-11
90/100 stars
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ca2 -free eagle’s minimal essential medium (emem)  (BioWhittaker Molecular Applications)
90
BioWhittaker Molecular Applications ca2 -free eagle’s minimal essential medium (emem)
WRS increases MMP activity in macrophage-conditioned media by a TNFα-dependent pathway. PMA-differentiated THP1-derived macrophages were treated for 24 h with recombinant human WRS (100 nm), Δ1–47 WRS (100 nm) as an analog of the MMP7 and MMP8 cleavage product, TNFα (40 ng/ml), or buffer with or without a mAb inhibitor of TNFα <t>(infliximab</t> (IN)) (100 ng/ml). MMPs in 20-fold–concentrated conditioned media were activated with 1 mm APMA, and cleavage of the quenched fluorescence peptide substrate Mca-Pro-Leu-Gly↓Leu-Dpa-Ala-Arg-NH2 was measured (scatter plots show means ± S.D., n = 3) in N = 3 independent experiments. Statistical significance was determined against buffer + infliximab using a one-way ANOVA with Dunnett's multiple comparison posttests. ***, p < 0.001; ns, not significant. Error bars represent S.D.
Ca2 Free Eagle’s Minimal Essential Medium (Emem), supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/free+ca+2/ca2++free+eagle%E2%80%99s+minimal+essential+medium++emem+/10__1074_slash_jbc__m301356200-52-2-10
Average 90 stars, based on 1 article reviews
ca2 -free eagle’s minimal essential medium (emem) - by Bioz Stars, 2026-11
90/100 stars
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endotoxin-free pbs without ca 2+ and 2  (BioWhittaker Molecular Applications)
90
BioWhittaker Molecular Applications endotoxin-free pbs without ca 2+ and 2
WRS increases MMP activity in macrophage-conditioned media by a TNFα-dependent pathway. PMA-differentiated THP1-derived macrophages were treated for 24 h with recombinant human WRS (100 nm), Δ1–47 WRS (100 nm) as an analog of the MMP7 and MMP8 cleavage product, TNFα (40 ng/ml), or buffer with or without a mAb inhibitor of TNFα <t>(infliximab</t> (IN)) (100 ng/ml). MMPs in 20-fold–concentrated conditioned media were activated with 1 mm APMA, and cleavage of the quenched fluorescence peptide substrate Mca-Pro-Leu-Gly↓Leu-Dpa-Ala-Arg-NH2 was measured (scatter plots show means ± S.D., n = 3) in N = 3 independent experiments. Statistical significance was determined against buffer + infliximab using a one-way ANOVA with Dunnett's multiple comparison posttests. ***, p < 0.001; ns, not significant. Error bars represent S.D.
Endotoxin Free Pbs Without Ca 2+ And 2, supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/free+ca+2/endotoxin+free+pbs+without+ca+2++and+2/pmc03416947-97-32-33
Average 90 stars, based on 1 article reviews
endotoxin-free pbs without ca 2+ and 2 - by Bioz Stars, 2026-11
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1 ml 37°c ca 2+ /mg 2+ -free hbss (with 1 mm egta)  (BioIVT Inc)
90
BioIVT Inc 1 ml 37°c ca 2+ /mg 2+ -free hbss (with 1 mm egta)
WRS increases MMP activity in macrophage-conditioned media by a TNFα-dependent pathway. PMA-differentiated THP1-derived macrophages were treated for 24 h with recombinant human WRS (100 nm), Δ1–47 WRS (100 nm) as an analog of the MMP7 and MMP8 cleavage product, TNFα (40 ng/ml), or buffer with or without a mAb inhibitor of TNFα <t>(infliximab</t> (IN)) (100 ng/ml). MMPs in 20-fold–concentrated conditioned media were activated with 1 mm APMA, and cleavage of the quenched fluorescence peptide substrate Mca-Pro-Leu-Gly↓Leu-Dpa-Ala-Arg-NH2 was measured (scatter plots show means ± S.D., n = 3) in N = 3 independent experiments. Statistical significance was determined against buffer + infliximab using a one-way ANOVA with Dunnett's multiple comparison posttests. ***, p < 0.001; ns, not significant. Error bars represent S.D.
1 Ml 37°C Ca 2+ /Mg 2+ Free Hbss (With 1 Mm Egta), supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/free+ca+2/1+ml+37+c+ca+2+++mg+2+++free+hbss++with+1+mm+egta+/pmc06596447-104-21-31
Average 90 stars, based on 1 article reviews
1 ml 37°c ca 2+ /mg 2+ -free hbss (with 1 mm egta) - by Bioz Stars, 2026-11
90/100 stars
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calcium and magnesium-free hanks’ balanced salt solution hbss  (Welgene inc)
90
Welgene inc calcium and magnesium-free hanks’ balanced salt solution hbss
WRS increases MMP activity in macrophage-conditioned media by a TNFα-dependent pathway. PMA-differentiated THP1-derived macrophages were treated for 24 h with recombinant human WRS (100 nm), Δ1–47 WRS (100 nm) as an analog of the MMP7 and MMP8 cleavage product, TNFα (40 ng/ml), or buffer with or without a mAb inhibitor of TNFα <t>(infliximab</t> (IN)) (100 ng/ml). MMPs in 20-fold–concentrated conditioned media were activated with 1 mm APMA, and cleavage of the quenched fluorescence peptide substrate Mca-Pro-Leu-Gly↓Leu-Dpa-Ala-Arg-NH2 was measured (scatter plots show means ± S.D., n = 3) in N = 3 independent experiments. Statistical significance was determined against buffer + infliximab using a one-way ANOVA with Dunnett's multiple comparison posttests. ***, p < 0.001; ns, not significant. Error bars represent S.D.
Calcium And Magnesium Free Hanks’ Balanced Salt Solution Hbss, supplied by Welgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/free+ca+2/ca2+++and+mg2++free+hank%E2%80%99s+balanced+saline+solution++hbss+/pmc06319133-66-7-13
Average 90 stars, based on 1 article reviews
calcium and magnesium-free hanks’ balanced salt solution hbss - by Bioz Stars, 2026-11
90/100 stars
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ca 2+ 2+ -free medium  (Cook Canada Inc)
90
Cook Canada Inc ca 2+ 2+ -free medium
WRS increases MMP activity in macrophage-conditioned media by a TNFα-dependent pathway. PMA-differentiated THP1-derived macrophages were treated for 24 h with recombinant human WRS (100 nm), Δ1–47 WRS (100 nm) as an analog of the MMP7 and MMP8 cleavage product, TNFα (40 ng/ml), or buffer with or without a mAb inhibitor of TNFα <t>(infliximab</t> (IN)) (100 ng/ml). MMPs in 20-fold–concentrated conditioned media were activated with 1 mm APMA, and cleavage of the quenched fluorescence peptide substrate Mca-Pro-Leu-Gly↓Leu-Dpa-Ala-Arg-NH2 was measured (scatter plots show means ± S.D., n = 3) in N = 3 independent experiments. Statistical significance was determined against buffer + infliximab using a one-way ANOVA with Dunnett's multiple comparison posttests. ***, p < 0.001; ns, not significant. Error bars represent S.D.
Ca 2+ 2+ Free Medium, supplied by Cook Canada Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/free+ca+2/ca+2++2+++free+medium/pmc03162057-72-9-16
Average 90 stars, based on 1 article reviews
ca 2+ 2+ -free medium - by Bioz Stars, 2026-11
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Image Search Results


WRS increases MMP activity in macrophage-conditioned media by a TNFα-dependent pathway. PMA-differentiated THP1-derived macrophages were treated for 24 h with recombinant human WRS (100 nm), Δ1–47 WRS (100 nm) as an analog of the MMP7 and MMP8 cleavage product, TNFα (40 ng/ml), or buffer with or without a mAb inhibitor of TNFα (infliximab (IN)) (100 ng/ml). MMPs in 20-fold–concentrated conditioned media were activated with 1 mm APMA, and cleavage of the quenched fluorescence peptide substrate Mca-Pro-Leu-Gly↓Leu-Dpa-Ala-Arg-NH2 was measured (scatter plots show means ± S.D., n = 3) in N = 3 independent experiments. Statistical significance was determined against buffer + infliximab using a one-way ANOVA with Dunnett's multiple comparison posttests. ***, p < 0.001; ns, not significant. Error bars represent S.D.

Journal: The Journal of Biological Chemistry

Article Title: Matrix metalloproteinases inactivate the proinflammatory functions of secreted moonlighting tryptophanyl-tRNA synthetase

doi: 10.1074/jbc.RA119.009584

Figure Lengend Snippet: WRS increases MMP activity in macrophage-conditioned media by a TNFα-dependent pathway. PMA-differentiated THP1-derived macrophages were treated for 24 h with recombinant human WRS (100 nm), Δ1–47 WRS (100 nm) as an analog of the MMP7 and MMP8 cleavage product, TNFα (40 ng/ml), or buffer with or without a mAb inhibitor of TNFα (infliximab (IN)) (100 ng/ml). MMPs in 20-fold–concentrated conditioned media were activated with 1 mm APMA, and cleavage of the quenched fluorescence peptide substrate Mca-Pro-Leu-Gly↓Leu-Dpa-Ala-Arg-NH2 was measured (scatter plots show means ± S.D., n = 3) in N = 3 independent experiments. Statistical significance was determined against buffer + infliximab using a one-way ANOVA with Dunnett's multiple comparison posttests. ***, p < 0.001; ns, not significant. Error bars represent S.D.

Article Snippet: To assess MMP activity in conditioned medium, THP1 M0 cells were cultured in phenol red–free, serum-free growth medium for 24 h with 10 μg/ml polymyxin B plus 100 n m WRS, TNFα 40 μg/ml, or PBS ± 100 μg/ml infliximab (Novus Biologicals, catalog number NBP2-52655).

Techniques: Activity Assay, Derivative Assay, Recombinant, Fluorescence, Comparison