free ca 2 Search Results


rabbit polyclonal anti xbp1  (Novus Biologicals)
92
Novus Biologicals rabbit polyclonal anti xbp1
Rabbit Polyclonal Anti Xbp1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tnfα neutralizing antibody infliximab  (Novus Biologicals)
94
Novus Biologicals tnfα neutralizing antibody infliximab
A) Schematic of neutrophil transwell migration assay. B) Migration of human primary neutrophils towards conditioned medium (CM) from A431 keratinocyte cultures. Individual symbols depict data using neutrophils from different donors (n=4). Data are shown as mean ± SEM. One-way ANOVA. C-D) ELISA measurements of selected chemokine levels in A431 CM (pg/ml). N=3 measurements for CXCL1 (2 technical replicates each), n=2 measurements for CXCL11 (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. E) Migration of human primary neutrophils towards A431 CM in the presence of CXCR2 antagonist and/or CXCR3 antagonist. Individual symbols depict data using neutrophils from different donors (n=4-6). Data are shown as mean ± SEM. Two-way ANOVA. F) ELISA measurements of <t>TNFα</t> levels in A431 CM (pg/ml). n=2 measurements (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. Acet, Acetone.
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inflixamab nbp2 52655 novus biologicals  (Novus Biologicals)
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Novus Biologicals inflixamab nbp2 52655 novus biologicals
A) Schematic of neutrophil transwell migration assay. B) Migration of human primary neutrophils towards conditioned medium (CM) from A431 keratinocyte cultures. Individual symbols depict data using neutrophils from different donors (n=4). Data are shown as mean ± SEM. One-way ANOVA. C-D) ELISA measurements of selected chemokine levels in A431 CM (pg/ml). N=3 measurements for CXCL1 (2 technical replicates each), n=2 measurements for CXCL11 (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. E) Migration of human primary neutrophils towards A431 CM in the presence of CXCR2 antagonist and/or CXCR3 antagonist. Individual symbols depict data using neutrophils from different donors (n=4-6). Data are shown as mean ± SEM. Two-way ANOVA. F) ELISA measurements of <t>TNFα</t> levels in A431 CM (pg/ml). n=2 measurements (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. Acet, Acetone.
Inflixamab Nbp2 52655 Novus Biologicals, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ca2+/mg2+ free buffered medium vitrolife g-pgd  (Vitrolife Inc)
90
Vitrolife Inc ca2+/mg2+ free buffered medium vitrolife g-pgd
A) Schematic of neutrophil transwell migration assay. B) Migration of human primary neutrophils towards conditioned medium (CM) from A431 keratinocyte cultures. Individual symbols depict data using neutrophils from different donors (n=4). Data are shown as mean ± SEM. One-way ANOVA. C-D) ELISA measurements of selected chemokine levels in A431 CM (pg/ml). N=3 measurements for CXCL1 (2 technical replicates each), n=2 measurements for CXCL11 (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. E) Migration of human primary neutrophils towards A431 CM in the presence of CXCR2 antagonist and/or CXCR3 antagonist. Individual symbols depict data using neutrophils from different donors (n=4-6). Data are shown as mean ± SEM. Two-way ANOVA. F) ELISA measurements of <t>TNFα</t> levels in A431 CM (pg/ml). n=2 measurements (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. Acet, Acetone.
Ca2+/Mg2+ Free Buffered Medium Vitrolife G Pgd, supplied by Vitrolife Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbs with ca++ /mg  (Mediatech)
90
Mediatech pbs with ca++ /mg
A) Schematic of neutrophil transwell migration assay. B) Migration of human primary neutrophils towards conditioned medium (CM) from A431 keratinocyte cultures. Individual symbols depict data using neutrophils from different donors (n=4). Data are shown as mean ± SEM. One-way ANOVA. C-D) ELISA measurements of selected chemokine levels in A431 CM (pg/ml). N=3 measurements for CXCL1 (2 technical replicates each), n=2 measurements for CXCL11 (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. E) Migration of human primary neutrophils towards A431 CM in the presence of CXCR2 antagonist and/or CXCR3 antagonist. Individual symbols depict data using neutrophils from different donors (n=4-6). Data are shown as mean ± SEM. Two-way ANOVA. F) ELISA measurements of <t>TNFα</t> levels in A431 CM (pg/ml). n=2 measurements (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. Acet, Acetone.
Pbs With Ca++ /Mg, supplied by Mediatech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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endotoxin-free pbs without ca 2+ and 2  (BioWhittaker Molecular Applications)
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BioWhittaker Molecular Applications endotoxin-free pbs without ca 2+ and 2
A) Schematic of neutrophil transwell migration assay. B) Migration of human primary neutrophils towards conditioned medium (CM) from A431 keratinocyte cultures. Individual symbols depict data using neutrophils from different donors (n=4). Data are shown as mean ± SEM. One-way ANOVA. C-D) ELISA measurements of selected chemokine levels in A431 CM (pg/ml). N=3 measurements for CXCL1 (2 technical replicates each), n=2 measurements for CXCL11 (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. E) Migration of human primary neutrophils towards A431 CM in the presence of CXCR2 antagonist and/or CXCR3 antagonist. Individual symbols depict data using neutrophils from different donors (n=4-6). Data are shown as mean ± SEM. Two-way ANOVA. F) ELISA measurements of <t>TNFα</t> levels in A431 CM (pg/ml). n=2 measurements (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. Acet, Acetone.
Endotoxin Free Pbs Without Ca 2+ And 2, supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apoplastic pool of free ca2  (Broadley James Corp)
90
Broadley James Corp apoplastic pool of free ca2
A) Schematic of neutrophil transwell migration assay. B) Migration of human primary neutrophils towards conditioned medium (CM) from A431 keratinocyte cultures. Individual symbols depict data using neutrophils from different donors (n=4). Data are shown as mean ± SEM. One-way ANOVA. C-D) ELISA measurements of selected chemokine levels in A431 CM (pg/ml). N=3 measurements for CXCL1 (2 technical replicates each), n=2 measurements for CXCL11 (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. E) Migration of human primary neutrophils towards A431 CM in the presence of CXCR2 antagonist and/or CXCR3 antagonist. Individual symbols depict data using neutrophils from different donors (n=4-6). Data are shown as mean ± SEM. Two-way ANOVA. F) ELISA measurements of <t>TNFα</t> levels in A431 CM (pg/ml). n=2 measurements (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. Acet, Acetone.
Apoplastic Pool Of Free Ca2, supplied by Broadley James Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ca2 -free eagle’s minimal essential medium (emem)  (BioWhittaker Molecular Applications)
90
BioWhittaker Molecular Applications ca2 -free eagle’s minimal essential medium (emem)
A) Schematic of neutrophil transwell migration assay. B) Migration of human primary neutrophils towards conditioned medium (CM) from A431 keratinocyte cultures. Individual symbols depict data using neutrophils from different donors (n=4). Data are shown as mean ± SEM. One-way ANOVA. C-D) ELISA measurements of selected chemokine levels in A431 CM (pg/ml). N=3 measurements for CXCL1 (2 technical replicates each), n=2 measurements for CXCL11 (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. E) Migration of human primary neutrophils towards A431 CM in the presence of CXCR2 antagonist and/or CXCR3 antagonist. Individual symbols depict data using neutrophils from different donors (n=4-6). Data are shown as mean ± SEM. Two-way ANOVA. F) ELISA measurements of <t>TNFα</t> levels in A431 CM (pg/ml). n=2 measurements (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. Acet, Acetone.
Ca2 Free Eagle’s Minimal Essential Medium (Emem), supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ca2+and mg2+-free phosphate-buffered saline (cmf-pbs)  (Nissui Pharmaceutical)
90
Nissui Pharmaceutical ca2+and mg2+-free phosphate-buffered saline (cmf-pbs)
A) Schematic of neutrophil transwell migration assay. B) Migration of human primary neutrophils towards conditioned medium (CM) from A431 keratinocyte cultures. Individual symbols depict data using neutrophils from different donors (n=4). Data are shown as mean ± SEM. One-way ANOVA. C-D) ELISA measurements of selected chemokine levels in A431 CM (pg/ml). N=3 measurements for CXCL1 (2 technical replicates each), n=2 measurements for CXCL11 (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. E) Migration of human primary neutrophils towards A431 CM in the presence of CXCR2 antagonist and/or CXCR3 antagonist. Individual symbols depict data using neutrophils from different donors (n=4-6). Data are shown as mean ± SEM. Two-way ANOVA. F) ELISA measurements of <t>TNFα</t> levels in A431 CM (pg/ml). n=2 measurements (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. Acet, Acetone.
Ca2+And Mg2+ Free Phosphate Buffered Saline (Cmf Pbs), supplied by Nissui Pharmaceutical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hanks’ balanced salt solution with ca 2+ and 2  (Corning Life Sciences)
90
Corning Life Sciences hanks’ balanced salt solution with ca 2+ and 2
A) Schematic of neutrophil transwell migration assay. B) Migration of human primary neutrophils towards conditioned medium (CM) from A431 keratinocyte cultures. Individual symbols depict data using neutrophils from different donors (n=4). Data are shown as mean ± SEM. One-way ANOVA. C-D) ELISA measurements of selected chemokine levels in A431 CM (pg/ml). N=3 measurements for CXCL1 (2 technical replicates each), n=2 measurements for CXCL11 (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. E) Migration of human primary neutrophils towards A431 CM in the presence of CXCR2 antagonist and/or CXCR3 antagonist. Individual symbols depict data using neutrophils from different donors (n=4-6). Data are shown as mean ± SEM. Two-way ANOVA. F) ELISA measurements of <t>TNFα</t> levels in A431 CM (pg/ml). n=2 measurements (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. Acet, Acetone.
Hanks’ Balanced Salt Solution With Ca 2+ And 2, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kbm-gold calcium and phenol-red free bullet kit  (Lonza)
90
Lonza kbm-gold calcium and phenol-red free bullet kit
A) Schematic of neutrophil transwell migration assay. B) Migration of human primary neutrophils towards conditioned medium (CM) from A431 keratinocyte cultures. Individual symbols depict data using neutrophils from different donors (n=4). Data are shown as mean ± SEM. One-way ANOVA. C-D) ELISA measurements of selected chemokine levels in A431 CM (pg/ml). N=3 measurements for CXCL1 (2 technical replicates each), n=2 measurements for CXCL11 (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. E) Migration of human primary neutrophils towards A431 CM in the presence of CXCR2 antagonist and/or CXCR3 antagonist. Individual symbols depict data using neutrophils from different donors (n=4-6). Data are shown as mean ± SEM. Two-way ANOVA. F) ELISA measurements of <t>TNFα</t> levels in A431 CM (pg/ml). n=2 measurements (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. Acet, Acetone.
Kbm Gold Calcium And Phenol Red Free Bullet Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ca 2 free ebss  (Irvine Scientific)
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Irvine Scientific ca 2 free ebss
A) Schematic of neutrophil transwell migration assay. B) Migration of human primary neutrophils towards conditioned medium (CM) from A431 keratinocyte cultures. Individual symbols depict data using neutrophils from different donors (n=4). Data are shown as mean ± SEM. One-way ANOVA. C-D) ELISA measurements of selected chemokine levels in A431 CM (pg/ml). N=3 measurements for CXCL1 (2 technical replicates each), n=2 measurements for CXCL11 (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. E) Migration of human primary neutrophils towards A431 CM in the presence of CXCR2 antagonist and/or CXCR3 antagonist. Individual symbols depict data using neutrophils from different donors (n=4-6). Data are shown as mean ± SEM. Two-way ANOVA. F) ELISA measurements of <t>TNFα</t> levels in A431 CM (pg/ml). n=2 measurements (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. Acet, Acetone.
Ca 2 Free Ebss, supplied by Irvine Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A) Schematic of neutrophil transwell migration assay. B) Migration of human primary neutrophils towards conditioned medium (CM) from A431 keratinocyte cultures. Individual symbols depict data using neutrophils from different donors (n=4). Data are shown as mean ± SEM. One-way ANOVA. C-D) ELISA measurements of selected chemokine levels in A431 CM (pg/ml). N=3 measurements for CXCL1 (2 technical replicates each), n=2 measurements for CXCL11 (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. E) Migration of human primary neutrophils towards A431 CM in the presence of CXCR2 antagonist and/or CXCR3 antagonist. Individual symbols depict data using neutrophils from different donors (n=4-6). Data are shown as mean ± SEM. Two-way ANOVA. F) ELISA measurements of TNFα levels in A431 CM (pg/ml). n=2 measurements (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. Acet, Acetone.

Journal: bioRxiv

Article Title: Keratin 17- and PKCα-dependent transient amplification of neutrophil influx after repeated stress to the skin

doi: 10.1101/2023.10.11.561954

Figure Lengend Snippet: A) Schematic of neutrophil transwell migration assay. B) Migration of human primary neutrophils towards conditioned medium (CM) from A431 keratinocyte cultures. Individual symbols depict data using neutrophils from different donors (n=4). Data are shown as mean ± SEM. One-way ANOVA. C-D) ELISA measurements of selected chemokine levels in A431 CM (pg/ml). N=3 measurements for CXCL1 (2 technical replicates each), n=2 measurements for CXCL11 (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. E) Migration of human primary neutrophils towards A431 CM in the presence of CXCR2 antagonist and/or CXCR3 antagonist. Individual symbols depict data using neutrophils from different donors (n=4-6). Data are shown as mean ± SEM. Two-way ANOVA. F) ELISA measurements of TNFα levels in A431 CM (pg/ml). n=2 measurements (4 technical replicates each). Data are shown as mean ± SEM. One-way ANOVA. Acet, Acetone.

Article Snippet: These inhibitors together with 1μg/ml of TNFα neutralizing antibody Infliximab (Novus Biologicals #NBP2-52655) do not alter neutrophil migration in response to fMLF, a potent neutrophil chemoattractant , suggesting that neutrophils can respond normally to other chemotactic cues in the presence of the inhibitors ( Suppl.

Techniques: Transwell Migration Assay, Migration, Enzyme-linked Immunosorbent Assay